Elicit: Benchmarks for Scientific Literature Review Performance (public)

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Benchmarks for Scientific Literature Review Performance (public)

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September 12, 2025

Which benchmarks measure performance on scientific literature review tasks?

Benchmarks for scientific literature review tasks include datasets like LitSearch, SciReviewGen, SWIFT‐Review, and RoBBR that measure performance across retrieval, generation, screening, extraction, and meta-evaluation areas.

Abstract

Benchmarks for scientific literature review tasks address five primary areas: retrieval, generation, screening, extraction, and meta-evaluation. Seventeen studies target retrieval using datasets such as LitSearch, CLEF TAR, CSMeD, SIGIR2017-PICO-Collection, FASS‐BSLR, RELISH, and ResearchArena. These studies report performance using metrics such as recall, precision, Mean Average Precision, normalized Discounted Cumulative Gain, and Work Saved over Sampling. Eleven studies focus on generation tasks—including review, table, or abstract writing—with benchmarks like SciReviewGen, ARXIV2TABLE, MSLR2022, ScholarQABench, and Manalyzer; performance is measured by ROUGE scores, hallucination rates, semantic coverage, and human ratings. Nine studies on screening tasks (using tools such as SWIFT‐Review and LGAR) report measures such as precision at high recall levels and WSS@95, while five studies on extraction or structured analysis (using RoBBR, EvidenceBench, and ECACT) employ metrics such as macro‐F1, accuracy, and Cohen’s kappa. One study adopts a meta‐evaluation benchmark using Elo ratings and inter‐annotator agreement. Overall, papers combine automated retrieval and overlap metrics with human evaluation methods to capture the multifaceted nature of performance in scientific literature review tasks.

Methods

We analyzed 40 sources from an initial pool of 1000, using 5 screening criteria. Each paper was reviewed for 4 key aspects that mattered most to the research question. More on methods

Papers identified with Elicit search

n = 1000

Papers screened using: Benchmark Focus, Computational Literature Review Systems, Beyond Methods Description Only, Literature Review Specific Application, Computational Component

n = 1000

Papers screened out

n = 960

Papers included for extraction

n = 40

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Paper search

Using your research question “Which benchmarks measure performance on scientific literature review tasks?”, we searched across over 126 million academic papers from the Semantic Scholar corpus. We retrieved the 1000 papers most relevant to the query.

Screening

We screened in sources based on their abstracts that met these criteria:

We considered all screening questions together and made a holistic judgement about whether to screen in each paper.

Data extraction

We asked a large language model to extract each data column below from each paper. We gave the model the extraction instructions shown below for each column.

Identify and describe the specific type of benchmark created in the study. Look for explicit statements about the benchmark’s purpose, focus, and unique characteristics.

Extraction guidelines:

Examples might include:

If no clear benchmark is described, write “Not applicable” or “No benchmark created”

Extract detailed information about the dataset used to create or evaluate the benchmark.

Look for:

Extraction guidelines:

Format examples:

Identify and describe the specific metrics used to assess benchmark performance.

Extraction guidelines:

Look for metrics such as:

Format examples:

Document the models or systems tested against the benchmark.

Extraction guidelines:

Look for:

Format examples:

Results

Characteristics of Included Studies

Study

Study Focus

Benchmark/Dataset Name

Literature Review Task Type

Evaluation Approach

Full text retrieved

Zhu et al., 2023

Hierarchical catalogue generation for literature reviews

HiCaD

Hierarchical catalogue generation

CEDS (semantic/structural similarity), CQE (informativeness)

Yes

Ajith et al., 2024

Literature search retrieval

LitSearch

Literature search/retrieval

Recall at 5, recall at 20, normalized Discounted Cumulative Gain at 10 (nDCG@10)

Yes

Kanoulas et al., 2019

Systematic review retrieval and ranking

CLEF 2019 e-Health Technology-Assisted Review (TAR)

Retrieval, ranking for systematic reviews

No mention found in abstract

No

Kasanishi et al., 2023

Automatic literature review generation

SciReviewGen

Literature review generation (summarization)

Recall-Oriented Understudy for Gisting Evaluation (ROUGE), human evaluation (relevance, coherence, informativeness, factuality)

Yes

Howard et al., 2016

Automated citation screening

SWIFT-Review datasets

Citation screening

Work Saved over Sampling at 95% (WSS@95%), precision at 95% recall

Yes

Wang et al., 2025a

Literature review table generation

ARXIV2TABLE

Table generation for literature reviews

Recall, precision, F1 for schema/cell/pairwise overlap

Yes

Kusa et al., 2023a

Outcome-based evaluation of systematic review automation

CLEF TAR 2019

Systematic review automation (retrieval)

Mean Average Precision (MAP), Recall at k%, WSS, Area Under the Curve (AUC), outcome difference

Yes

Kusa et al., 2023b

Automated citation screening

CSMeD, CSMeD-FT

Citation/full-text screening

True Negative Rate at 95% (TNR@95%), normalized Precision at 95% (nP@95%), nDCG@10, MAP, macro-precision/recall/F1

Yes

Scells et al., 2017

Retrieval for systematic reviews

SIGIR2017-PICO-Collection

Retrieval, screening prioritization

Precision-recall, F-beta, WSS, Average Precision (AP), nDCG, MAP

Yes

Budau and Ensan, 2024

Automated study search for biomedical systematic literature reviews

FASS-BSLR

Study search (retrieval, Boolean query generation)

Precision, Recall, NDCG, MAP, Recall at 1000

No

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Note:All abbreviations and acronyms are defined at first use in the table or in this note. “No mention found” indicates that we did not find mention of the information in the available abstract or full text.

Distribution of Literature Review Automation Tasks

Distribution of Evaluation Approaches

Some studies addressed multiple task types or used multiple evaluation approaches. We did not find mention of evaluation approach for 6 studies, and task type information was sometimes ambiguous due to overlapping or unclear descriptions.


Thematic Analysis

Benchmarks for Literature Retrieval and Screening Tasks

Benchmarks in this category focus on identifying, retrieving, and prioritizing relevant literature for systematic reviews or evidence synthesis. Key benchmarks include:

Benchmarks for Literature Summarization and Synthesis Tasks

This theme covers benchmarks designed to evaluate the ability of models to generate summaries, reviews, or structured tables from collections of scientific papers. Notable benchmarks include:

Benchmarks for Structured Literature Analysis Tasks

Structured analysis tasks include risk of bias assessment, data element extraction, compliance and traceability, and meta-evaluation. Key benchmarks:

Evaluation Methodologies Across Benchmarks

Benchmark Category

Primary Metrics

Evaluation Method

Performance Measurement Focus

Retrieval/Screening

Recall, precision, Mean Average Precision, normalized Discounted Cumulative Gain, Work Saved over Sampling, Average Precision

Automated scoring, comparison to human or baseline models

Relevance, workload reduction, ranking effectiveness

Summarization/Synthesis

ROUGE, human ratings, semantic coverage, hallucination rate

Automated metrics, human evaluation

Informativeness, factuality, coherence, citation accuracy

Structured Analysis

Macro-F1, accuracy, Cohen’s kappa, composite scores (ECACT)

Automated scoring, statistical analysis, human validation

Bias, compliance, extraction accuracy, traceability

Meta-evaluation

Elo ratings, accuracy, agreement

Human preference voting, model-based evaluator assessment

Alignment with human judgment, evaluator reliability

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Summary of Evaluation Approaches:

References

Jianyou Wang, Weili Cao, Longtian Bao, Youze Zheng, Gil Pasternak, and 4 more\ (2024).Measuring Risk of Bias in Biomedical Reports: The RoBBR Benchmark. arXiv.org

Kun Zhu, Xiaocheng Feng, Xiachong Feng, Yin Wu, Bing Qin\ (2023).Hierarchical Catalogue Generation for Literature Review: A Benchmark. Conference on Empirical Methods in Natural Language Processing

Anirudh Ajith, Mengzhou Xia, Alexis Chevalier, Tanya Goyal, Danqi Chen, and 1 more\ (2024).LitSearch: A Retrieval Benchmark for Scientific Literature Search. Conference on Empirical Methods in Natural Language Processing

David W. Brett, Anniek Myatt\ (2025).Patience is all you need! An agentic system for performing scientific literature review. arXiv.org

E. Kanoulas, Dan Li, L. Azzopardi, R. Spijker\ (2018).CLEF 2017 Technologically Assisted Reviews in Empirical Medicine Overview. Conference and Labs of the Evaluation Forum

Lucy Lu Wang, Jay DeYoung, Byron Wallace\ (2022).Overview of MSLR2022: A Shared Task on Multi-document Summarization for Literature Reviews. SDP

E. Kanoulas, Dan Li, Leif Azzopardi, René Spijker\ (2019).CLEF 2019 Technology Assisted Reviews in Empirical Medicine Overview. Conference and Labs of the Evaluation Forum

E. Kanoulas, Dan Li, Leif Azzopardi, René Spijker\ (2018).CLEF 2018 Technologically Assisted Reviews in Empirical Medicine Overview. Conference and Labs of the Evaluation Forum

Tetsu Kasanishi, Masaru Isonuma, Junichiro Mori, I. Sakata\ (2023).SciReviewGen: A Large-scale Dataset for Automatic Literature Review Generation. Annual Meeting of the Association for Computational Linguistics

Brian E. Howard, Jason R. Phillips, Kyle Miller, Arpit Tandon, D. Mav, and 8 more\ (2016).SWIFT-Review: a text-mining workbench for systematic review. Systematic Reviews

Weiqi Wang, Jiefu Ou, Yangqiu Song, Benjamin Van Durme, Daniel Khashabi\ (2025).Can LLMs Generate Tabular Summaries of Science Papers? Rethinking the Evaluation Protocol. arXiv.org

Wojciech Kusa, G. Zuccon, Petr Knoth, A. Hanbury\ (2023).Outcome-based Evaluation of Systematic Review Automation. International Conference on the Theory of Information Retrieval

Wojciech Kusa, Óscar E. Mendoza, Matthias Samwald, Petr Knoth, Allan Hanbury\ (2023).CSMeD: Bridging the Dataset Gap in Automated Citation Screening for Systematic Literature Reviews. Neural Information Processing Systems

Harrisen Scells, G. Zuccon, B. Koopman, Anthony J Deacon, L. Azzopardi, and 1 more\ (2017).A Test Collection for Evaluating Retrieval of Studies for Inclusion in Systematic Reviews. Annual International ACM SIGIR Conference on Research and Development in Information Retrieval

Leandra Budau, F. Ensan\ (2024).Fully Automated Scholarly Search for Biomedical Systematic Literature Reviews. IEEE Access

Yilun Zhao, Kaiyan Zhang, Tiansheng Hu, Sihong Wu, Ronan Le Bras, and 13 more\ (2025).SciArena: An Open Evaluation Platform for Foundation Models in Scientific Literature Tasks. arXiv.org

Joshua Morriss, Tod Brindle, Jessica Bah Rösman, Daniel Reibsamen, Andreas Enz\ (2024).The Literature Review Network: An Explainable Artificial Intelligence for Systematic Literature Reviews, Meta-analyses, and Method Development. arXiv.org

Christian Jaumann, Andreas Wiedholz, Annemarie Friedrich\ (2025).LGAR: Zero-Shot LLM-Guided Neural Ranking for Abstract Screening in Systematic Literature Reviews. Annual Meeting of the Association for Computational Linguistics

Mohammadreza Pourreza, F. Ensan\ (2022).Towards semantic-driven boolean query formalization for biomedical systematic literature reviews. Int. J. Medical Informatics

Maxime Gobin, Muriel Gosnat, Seindé Toure, Lina Faik, Joel Belafa, and 2 more\ (2025).From data extraction to analysis: a comparative study of ELISE capabilities in scientific literature. Frontiers in Artificial Intelligence

Shubham Agarwal, Gaurav Sahu, Abhay Puri, I. Laradji, K. Dvijotham, and 3 more\ (2024).LitLLMs, LLMs for Literature Review: Are we there yet?. Trans. Mach. Learn. Res.

Peter Brown, Ameya Sadguru Kulkarni, Osama Refai, Yaoqi Zhou, Abd Al-Bar Al-Farha\ (2019).Large expert-curated database for benchmarking document similarity detection in biomedical literature search. Database J. Biol. Databases Curation

Christopher Polzak, Alejandro Lozano, Min Woo Sun, James Burgess, Yuhui Zhang, and 2 more\ (2025).Can Large Language Models Match the Conclusions of Systematic Reviews?. arXiv.org

Xuemei Tang, Xufeng Duan, Zhenguang G. Cai\ (2024).Large Language Models for Automated Literature Review: An Evaluation of Reference Generation, Abstract Writing, and Review Composition

Tanja Bekhuis, Eugene Tseytlin, K. Mitchell, Dina Demner-Fushman\ (2014).Feature Engineering and a Proposed Decision-Support System for Systematic Reviewers of Medical Evidence. PLoS ONE

Michael D. Skarlinski, Sam Cox, Jon M. Laurent, James D. Braza, Michaela M. Hinks, and 4 more\ (2024).Language agents achieve superhuman synthesis of scientific knowledge. arXiv.org

A. Lozano, Scott L. Fleming, Chia-Chun Chiang, Nigam H. Shah\ (2023).Clinfo.ai: An Open-Source Retrieval-Augmented Large Language Model System for Answering Medical Questions using Scientific Literature. Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing

Grace E. Lee, Aixin Sun\ (2018).Seed-driven Document Ranking for Systematic Reviews in Evidence-Based Medicine. Annual International ACM SIGIR Conference on Research and Development in Information Retrieval

Alon Gorenshtein, Kamel Shihada, Moran Sorka, Dvir Aran, Shahar Shelly\ (2025).LITERAS: Biomedical literature review and citation retrieval agents. Comput. Biol. Medicine

Gaelen Adam, Melinda Davies, Jerusha George, Eduardo L Caputo, Ja Mai Htun, and 7 more\ (2025).Machine Learning Tools To (Semi-)Automate Evidence Synthesis: A Rapid Review and Evidence Map

Aditya Nagori, Ricardo Accorsi Casonatto, Ayush Gautam, Abhinav Manikantha Sai Cheruvu, Rishikesan Kamaleswaran\ (2025).Open-Source Agentic Hybrid RAG Framework for Scientific Literature Review

G. Adam, Melinda Davies, Jerusha George, Eduardo L Caputo, Ja Mai Htun, and 7 more\ (2025).Machine Learning Tools To (Semi-) Automate Evidence Synthesis

Akari Asai, Jacqueline He, Rulin Shao, Weijia Shi, Amanpreet Singh, and 20 more\ (2024).OpenScholar: Synthesizing Scientific Literature with Retrieval-augmented LMs. arXiv.org

Mingxuan Du, Benfeng Xu, Chiwei Zhu, Xiaorui Wang, Zhendong Mao\ (2025).DeepResearch Bench: A Comprehensive Benchmark for Deep Research Agents. arXiv.org

Hao Kang, Chenyan Xiong\ (2024).ResearchArena: Benchmarking LLMs' Ability to Collect and Organize Information as Research Agents. arXiv.org

Zifeng Wang, Qiao Jin, Jiacheng Lin, Junyi Gao, Jathurshan Pradeepkumar, and 4 more\ (2025).TrialPanorama: Database and Benchmark for Systematic Review and Design of Clinical Trials. arXiv.org

Jianyou Wang, Weili Cao, Kaicheng Wang, Xiaoyue Wang, Ashish Dalvi, and 11 more\ (2025).EvidenceBench: A Benchmark for Extracting Evidence from Biomedical Papers. arXiv.org

Wanghan Xu, Wenlong Zhang, Fenghua Ling, Ben Fei, Yusong Hu, and 4 more\ (2025).Manalyzer: End-to-end Automated Meta-analysis with Multi-agent System. arXiv.org

Jingcheng Du, Dong Wang, Bin Lin, Long He, Liang-chin Huang, and 5 more\ (2025).Use of deep learning-based NLP models for full-text data elements extraction for systematic literature review tasks. Scientific Reports

Hao Kang, Chenyan Xiong\ (2024).ResearchArena: Benchmarking Large Language Models' Ability to Collect and Organize Information as Research Agents

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From data extraction to analysis: a comparative study of ELISE capabilities in scientific literature

Maxime Gobin, Muriel Gosnat, Seindé Toure, Lina Faik, Joel Belafa, Antoine Villedieu de Torcy, Florence Armstrong

Frontiers in Artificial Intelligence·

2025·

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Benchmark Type

- Literature metadata extraction benchmark - Scientific article comprehension benchmark - Insight generation benchmark - Regulatory compliance benchmark

Benchmark Dataset Characteristics

- Total number of items in the dataset: 9 articles - Types of items: Research papers - Domain or field of research covered: Biomedical research (experimental and applied) - Data collection method: Not explicitly mentioned - Source of data items: Not explicitly mentioned

Evaluation Metrics

- ECACT score: A composite score assessing AI reliability in scientific literature analysis, regulatory reporting, and clinical documentation. - Extraction: Assesses the ability to accurately identify and retrieve key bibliographic elements. - Comprehension: Evaluates the ability to interpret and structure key arguments, conclusions, and methodological aspects. - Analysis: Assesses the ability to engage in critical reasoning, summarize key findings, identify study limitations, and generate meaningful insights. - Compliance: Evaluates whether AI responses strictly adhere to document content and follow required guidelines. - Traceability: Assesses whether AI tools correctly highlight relevant sections used in their answers. - Statistical analysis: Uses one-way ANOVA, two-way ANOVA, and post-hoc Tukey HSD tests to determine significant differences in performance scores. - Sensitivity analysis: Conducted to evaluate the robustness of the ECACT scoring framework.

Benchmark Models and Comparisons

- Models/System evaluated: ChatGPT, ELISE, SciSpace/Typeset, Humata, Epsilon - Performance relative to benchmark: ELISE consistently outperforms others in extraction, comprehension, and analysis. ChatGPT is strong in data retrieval but weak in complex analysis. Epsilon, Humata, and SciSpace/Typeset show moderate performance with variability. - Comparative analysis: ELISE (87.50%) and ChatGPT (86.99%) are top performers in extraction. ELISE outperforms others in comprehension and analysis.

The exponential growth of scientific literature presents challenges for pharmaceutical, biotechnological, and Medtech industries, particularly in regulatory documentation, clinical research, and systematic reviews. Ensuring accurate data extraction, literature synthesis, and compliance with industry standards require AI tools that not only streamline workflows but also uphold scientific rigor. This study evaluates the performance of AI tools designed for bibliographic review, data extraction, and scientific synthesis, assessing their impact on decision-making, regulatory compliance, and research productivity. The AI tools assessed include general-purpose models like ChatGPT and specialized solutions such as ELISE (Elevated LIfe SciencEs), SciSpace/Typeset, Humata, and Epsilon. The evaluation is based on three main criteria: Extraction, Comprehension, and Analysis with Compliance and Traceability (ECACT) as additional dimensions. Human experts established reference benchmarks, while AI Evaluator models ensure objective performance measurement. The study introduces the ECACT score, a structured metric assessing AI reliability in scientific literature analysis, regulatory reporting and clinical documentation. Results demonstrate that ELISE consistently outperforms other AI tools, excelling in precise data extraction, deep contextual comprehension, and advanced content analysis. ELISE’s ability to generate traceable, well-reasoned insights makes it particularly well-suited for high-stakes applications such as regulatory affairs, clinical trials, and medical documentation, where accuracy, transparency, and compliance are paramount. Unlike other AI tools, ELISE provides expert-level reasoning and explainability, ensuring AI-generated insights align with industry best practices. ChatGPT is efficient in data retrieval but lacks precision in complex analysis, limiting its use in high-stakes decision-making. Epsilon, Humata, and SciSpace/Typeset exhibit moderate performance, with variability affecting their reliability in critical applications. In conclusion, while AI tools such as ELISE enhance literature review, regulatory writing, and clinical data interpretation, human oversight remains essential to validate AI outputs and ensure compliance with scientific and regulatory standards. For pharmaceutical, biotechnological, and Medtech industries, AI integration must strike a balance between automation and expert supervision to maintain data integrity, transparency, and regulatory adherence.

. These tools leverage advanced natural language processing (NLP) techniques to streamline traditionally manual tasks, such as literature reviews and data extraction (Fabiano et al., 2024). The development of sophisticated AI algorithms, fueled by increased computational power and growing data availability, has revolutionized how researchers and industry professionals interact with scientific literature (Zahra et al., 2024).

AI-driven literature analysis tools can be broadly categorized into general-purpose models and specialized scientific models, each serving distinct roles in research. General-purpose AI models, such as OpenAI's ChatGPT, are designed for versatility, able to handle a wide array of tasks across various domains. These models excel in summarizing and interpreting general information, offering researchers a flexible tool for diverse inquiries (Ning et al., 2024). In contrast, specialized tools like ELISE (the tool we developed at Biolevate), SciSpace (ex-Typeset), Humata, and Epsilon are tailored to meet the specific needs of scientific and biomedical research, including pharmaceutical, biotech and Medtech applications (Zahra et al., 2024;Tsirmpas et al., 2024). These applications focus on precision and relevance, employing advanced NLP techniques to extract and analyze data with a high level of specificity for regulated and highly technical fields. While general-purpose tools provide broad applicability, specialized tools ensure accuracy and domain relevance, crucial for rigorous academic inquiry.

More specifically, ChatGPT excels in conversational interactions, aiding in literature reviews and hypothesis generation (Khalifa and Ibrahim, 2024). SciSpace/Typeset enhances literature exploration with intuitive interfaces and robust search capabilities (SciSpace, 2024). Humata focuses on AI-driven text analysis, efficiently summarizing large volumes of data (Humata, 2024) . Epsilon identifies research gaps, suggesting innovative directions (Epsilon, 2024). Meanwhile, ELISE advances document analysis with sophisticated NLP and data extraction techniques, addressing the growing need for efficient data management in research (ELISE, 2024) .

Together, these AI tools enhance research quality and efficiency, facilitating a more comprehensive understanding of complex scientific literature and accelerating the pace of discovery by reducing the time required for knowledge synthesis (Borah et al., 2017;Chakraborty et al., 2024;Mehta et al., 2024). They support key industry processes, from early-stage drug discovery to regulatory documentation preparation, helping companies navigate vast and evolving scientific landscape (Agrawal et al., 2024). For pharmaceutical, biotech, and Medtech companies, AI tools can improve literature surveillance, support regulatory submissions (e.g., EMA/FDA filings), and optimize knowledge management for evidence-based decision-making.

Despite these advantages, integrating AI into scientific research is not without challenges, particularly in ensuring the reliability and consistency of AI-generated insights. A significant issue is the variability in AI-generated responses, which stems from the diverse methodologies and algorithms employed by different tools (Bolaños et al., 2024). This inconsistency complicates the standardization of evaluations, as outputs can vary in quality and relevance. Additionally, the lack of traceability in AI-generated insights raises concerns about the validity of interpretations, particularly in fields requiring high precision, such as biomedical and clinical research (Agrawal et al., 2024;Danler et al., 2024). Furthermore, the absence of a standardized framework for evaluating AI tools poses a threat to research reliability and validity (Ning et al., 2024;Sharma and Ruikar, 2024).

Therefore, there is a pressing need for rigorous and standardized evaluations to ensure that AI tools contribute effectively and reliably to scientific research, safeguarding the rigor and integrity of research outcomes.

This study aims to provide the scientific community and industry stakeholders with a clearer understanding of the potential and limitations of AI-driven literature analysis technologies. It also introduces a structured and independent methodology based on three main criteria (Extraction, Comprehension, and Analysis), to evaluate these applications, including ELISE, a novel AI tool integrating NLP and retrieval strategies to enhance workflows, improve regulatory compliance and optimize R&D processes for pharma, biotech and Medtech organizations.

2.1Selected articles

To ensure a comprehensive and exhaustive evaluation, a diverse selection of scientific articles was chosen, covering different disciplines and study types. The selected articles are detailed in Table 1 .

The current selection emphasized experimental and applied biomedical literature to stress-test AI tools in high-rigor contexts. Future work will include broader disciplinary representation to assess generalizability. This initial dataset includes articles in both English and French, allowing us to test cross-lingual consistency of the ECACT scoring framework. However, broader validation across other languages and domains remains a priority for future work.

The sample was restricted to nine articles to allow for a controlled proof-of-concept analysis. This scale enabled full evaluation across five criteria and three evaluators per article. However, broader validation will require expansion to larger datasets.

2.2.1AI tools

Among the most popular, several AI-driven literature analysis tools were selected based on their ability to extract, comprehend, and analyze scientific content. Table 2A summarizes their main characteristics.

2.2.2AI evaluator models

To assess the performance of the AI tools, independent AI Evaluator models were used, as described in Table 2B . To ensure a neutral and reproducible evaluation process, we selected three independent, high-performing AI models to act as evaluators: GPT-4o (OpenAI), Claude 3.5 Sonnet (Anthropic), and o1 Preview (OpenAI).

These models were intentionally chosen from distinct technical ecosystems and were not used at any stage in the development, training, or fine-tuning of the ELISE engine. This separation was critical to avoid any potential overlap or bias arising from shared components.

The selected models are known for their advanced reasoning and summarization capabilities, making them suitable for structured judgment across multiple scientific domains. Their inclusion was further motivated by public availability, multilingual support, and Each model performed evaluations independently, using randomized prompts under both anonymized and non-anonymized conditions, and their scores were subsequently averaged to control for evaluator-specific bias.

2.2.3Bias identification and model selection

To identify potential biases in AI-generated evaluations, we conducted a control assessment using Article 5 (Jones et al., 2021) on the Comprehension and Analysis criteria.

-AI tools were evaluated under two conditions: with their identities revealed and anonymized. -A comparison of the scoring results under both conditions allowed us to assess any bias in evaluator decisions.

To ensure transparency, AI tool responses were evaluated under both identified and anonymized conditions. For anonymized evaluations, tool names were replaced with neutral codes (Tool A, Tool B, etc.), and the order of responses was randomized. The evaluators were not informed of the anonymization. This protocol was repeated for all three evaluator models, and scoring results were then compared across both conditions to assess potential bias. Supplementary Table 1 presents the comparative results.

2.3Evaluation criteria

To objectively assess the performance of AI tools, three primary criteria were selected: Extraction, Comprehension, and Analysis. Each criterion was evaluated through a structured questionnaire described in Table 3 .

To limit subjectivity in open-ended tasks such as summarization, each question was paired with an expected response profile. Evaluators followed a standardized 10-point rubric (see Supplementary Table 3 ), and were prompted to assess semantic fidelity, relevance, and completeness rather than superficial similarity. For comparison-based judgments, evaluators used structured prompts to evaluate content quality and alignment with the source article.

2.4Evaluation protocol and scoring

Before evaluation, the questions listed in Table 3 were submitted to each AI tool for every article and every evaluation criterion. The queries were conducted between October 2024 and January 2025, and all responses were collected. The full evaluation process was carried out in January 2025.

2.4.1.1Extraction criterion

-Each response was manually checked by a human reviewer against expected data. -Each answer was scored out of 1, with partial credit assigned proportionally based on the number of correctly retrieved elements.

For instance, if an article was authored by three individuals and the AI tool retrieved only one name, the response received a score of 0.33/1. Similarly, if a study had four different sponsors but only two were correctly identified, the response was scored 0.5/1. -For each article and each AI tool, a percentage score was calculated by summing the points obtained across all questions (see Figure 1A ). -First, three independent human experts answered the questions, without knowing the answers of the AIs, to establish a reference baseline. -Second, two different evaluations were performed using automated evaluation prompts provided in Supplementary Figures 1A, B: i Each AI Evaluator model (presented in Table 2B ) assessed AI-generated responses against human-defined reference answers. ii AI Evaluator models independently assessed the responses based solely on document content, without human answers, to evaluate autonomous interpretability of AI-generated content.

-Each response was scored out of 10 by the AI Evaluator models and justified by them. -For each article and each AI tool, a percentage score was calculated by summing the points obtained across all questions (see Figure 1B ).

2.4.1.3Final performance assessment

-To assess global performance, the average score obtained across all evaluated articles was calculated for each AI tool. -Additionally, the standard deviation of scores was determined for each AI tool to measure variability in performance across different articles.

2.4.1.4Compliance and traceability scoring

-Compliance score (0-10): evaluates if AI responses strictly adhered to the document content and followed the required guidelines. -Traceability score (0-10): assesses whether AI tools correctly highlighted the relevant sections used in their answers.

2.4.1.5Final ECACT score calculation

A weighed global score (ECACT score) proposition was created to reflect the importance of each evaluation criterion:

Where E = Extraction, C = Comprehension, A = Analysis, C comp = Compliance, and T = Traceability, leading to a final score out of 80 points.The weighting system was designed to reflect the relative importance of each criterion in regulated scientific contexts. Analysis and Traceability were prioritized (×2.0) due to their implications for interpretability and compliance, particularly in biomedical and regulatory workflows. A sensitivity analysis exploring alternative weighting schemes is provided in Supplementary Table 2 .

2.5Statistical analysis

To ensure statistical robustness and objectivity in comparing the performance of AI tools, a comprehensive statistical framework was applied. All statistical analyses were conducted by a trained statistician (LF, co-author).

First, we performed a one-way ANOVA for each evaluation criterion (Extraction, Comprehension, Analysis) to determine whether differences in performance scores between AI tools were statistically significant. The assumption of homogeneity of variances was assessed using Levene's test, which evaluates whether the variances across groups are equal. A non-significant result (p > 0.05) confirmed that the assumption was met, allowing the use of standard ANOVA procedures.

Second, a two-way ANOVA was carried out to investigate both the main effects and their interaction effects between AI tool identity and evaluation criteria, thereby assessing whether certain models performed differently depending on the evaluation criterion.

Third, we applied post-hoc Tukey Honestly Significant Difference (HSD) tests to identify which pairs of AI tools showed statistically significant differences. This test was chosen for its robustness to multiple comparisons and its suitability for evaluating grouped means.

Additionally, to support transparency and explore the robustness of the ECACT scoring framework, a sensitivity analysis was conducted. Alternative weighting schemes (e.g., equal weights, compliance-prioritized, comprehension-focused) were applied to assess how different weight configurations impacted the final AI tool rankings. Results showed that while some mid-ranking positions Extraction (A) and comprehension/analysis (B) evaluation protocol. shifted, the top-performing (ELISE) and lowest-performing tools remained consistent across weighting conditions (see Supplementary Table 2 and Supplementary Figure 4 ). All analyses were performed using Python version 3.11.9 and validated independently (statsmodels v.0.14.4 and scipy v.1.14.1 libraries). A p-value threshold of 0.05 was used for significance across all tests.

The full evaluation dataset, question prompts, and scoring rubrics are available in the accompanying GitHub repository. 1

3Results

To provide the scientific community with a clearer understanding of the capabilities and limitations of AI-driven literature analysis applications, we developed a structured evaluation methodology based on three key criteria (Extraction, Comprehension and Analysis). Each of these criteria plays a critical role in assessing the overall effectiveness of AI tools in scientific research.

The evaluation process was conducted by analyzing the responses provided by each AI tool to a predefined set of questions (Table 3 ) across multiple scientific articles (Table 1 ). The articles were selected to represent a diverse range of disciplines and study types, ensuring a broad assessment of AI tool performance in different editorial contexts. Additionally, two equivalent articles (one in French and the other in English) were included in the dataset to investigate potential variations in AI performance due to language differences.

3.1Extraction performance

The first evaluation criterion, Extraction, assessed the ability of AI tools to accurately identify and retrieve key bibliographic elements such as author names, publication dates, study types, and other fundamental metadata. These elements are essential for organizing, referencing, and citing scientific work. The questions related to this criterion and their expected responses are detailed in Table 3 , with answers consisting exclusively of factual data. For each article and each question, the expected number of correct data points was predefined and AI-generated responses were manually evaluated for completeness and accuracy. Each response was assigned a score of 1 point, with proportional credit awarded when only a subset of the expected data was correctly retrieved (data not shown). Then a percentage score was calculated by summing the points obtained across all questions for each article and a global score is obtained for each AI tool.

The results of the Extraction evaluation are presented in Figure 2 , illustrating the performance of each AI tool across different articles as well as the global average performance. ChatGPT (Figure 2A ) and ELISE (Figure 2B ) demonstrate the highest extraction efficiency, consistently achieving scores above 80%, with a minimal variation across articles. In contrast, Epsilon (Figure 2C ), Humata (Figure 2D ), and SciSpace/Typeset (Figure 2E ) exhibited more variable performance, generally ranging between 60 and 70%, with significant fluctuations 1 https://github.com/Biolevate/SL-EVAL-ECACT depending on the articles. The global performance average (Figure 2F ) demonstrates that ELISE (87.50%) and ChatGPT (86.99%) were the most effective tools in extracting standard metadata, significantly outperforming Humata (52.00%), SciSpace/Typeset (49.10%) and Epsilon (37.41%). Statistical analysis revealed significant differences, with ELISE demonstrating a superior performance compared to Humata (p£0.01), SciSpace/Typeset (p£0.001), and Epsilon (p£0.001).

3.2Selection of evaluation models

To further assess the performance of AI tools beyond simple data extraction, we focused on two complex evaluation criteria: Comprehension and Analysis. These criteria require structured, explanatory and context-aware answers, which introduce significant variability in both quality and quantity. Such complexity makes human evaluation challenging, as scoring responses may be influenced by subjectivity and cognitive biases. To mitigate these risks, we established a set of calibrated guidelines (see Table 3 ) and opted for an AI-based evaluation approach.

A multi-model, independent evaluation was conducted to systematically assess AI tools performance on the Comprehension and Analysis criteria. To ensure robustness, a single reference article (Article 6 - Jones et al., 2021) was used in this evaluation. The primary objective was to determine whether AI Evaluator models introduced biases when grading responses, particularly by comparing identified and anonymized AI tool responses.

The evaluation was conducted using three AI Evaluator models: Claude 3.5 Sonnet, GPT-4o, and o1 Preview (Table 2B ). The responses from AI tools were processed separately by each evaluator, and their scores were then averaged (Figure 3 ).

Results indicate minimal variation between evaluations of identified and anonymized answers, demonstrating that the AI Evaluator models were not influenced by the identity of the AI tools being assessed. This consistency supports the reliability and objectivity of the evaluation framework.

The comparison between identified and anonymized scoring (see Supplementary Figure 1 ) revealed minimal variation for most tools, confirming that AI Evaluator models were not significantly influenced by tool identities. This supports the robustness and neutrality of the evaluation framework.

However, one notable discrepancy was observed in the evaluation of ChatGPT's responses, where the scores provided by Claude 3.5 Sonnet diverged from those of GPT-4o and o1 Preview. To maintain fairness and accuracy in scoring, as well as to ensure a balanced evaluation, the final assessment of AI tools was determined by incorporating the average results from all three AI Evaluator models. This approach minimizes potential biases and ensures that the final evaluation reflects a comprehensive and standardized assessment of AI-driven comprehension and analysis capabilities.

3.3Comprehension

The second evaluation criterion, Comprehension, aimed to assess the AI tools' ability to interpret and structure key arguments, conclusions and methodological aspects of scientific articles. This criterion is critical for determining how well AI models can process complex scientific content and provide accurate and coherent summaries.

To ensure an objective evaluation, a reference answer set was established by a panel of three human experts, defining expected responses for each question in every article. Then, the AI-generated responses were assessed by AI Evaluator models, which compared them to the human-defined references. Each response was scored on a 10-point scale, with justifications provided by the AI Evaluator models. The scores obtained for each AI tool, AI Evaluator model, and article were averaged (data not shown). The overall final score for each AI tool and article were averaged across the different evaluators and the results are presented in Figure 4 .

ELISE (Figure 4B ) demonstrated the highest performance in Comprehension, with scores consistently exceeding 8.0, highlighting its ability to process and synthesize scientific information effectively. In contrast, Epsilon (Figure 4C ), ChatGPT (Figure 4A ) and SciSpace/ Typeset (Figure 4E ) exhibited moderate performance, with scores ranging between 7.0 and 8.0. Humata (Figure 4D ) displayed greater variability with scores fluctuating between 5.0 and 8.0, indicating inconsistencies in its comprehension capabilities. The global evaluation (Figure 4F ) demonstrated that ELISE and Epsilon outperformed ChatGPT, Humata, and SciSpace/Typeset with final scores of: 8.23, 7.56, 7.48, 7.46 and 6.66, respectively. Statistical analysis demonstrated significant differences, reinforcing ELISE's superior comprehension capabilities compared to all other models.

3.4Analysis

The final evaluation criterion, Analysis, focused on assessing each AI tool's ability to engage in critical reasoning, summarize key findings, identify study limitations, and generate meaningful insights. ELISE (Figure 5B ) achieved the highest Analysis performance, with scores ranging between 7.0 and 9.0, demonstrating strong critical reasoning capabilities. In contrast, ChatGPT (Figure 5A ), SciSpace/ Typeset (Figure 5E ), Epsilon (Figure 5C ) and Humata (Figure 5D ), exhibited lower efficiency, with scores fluctuating between 4.5 and 8.0. Greater performance variability was observed in Humata, SciSpace/ Typeset and ChatGPT show greater variability in performance compared to Epsilon and ELISE, suggesting inconsistencies in their ability to generate structured and insightful interpretations.

The global performance evaluation (Figure 5F ) demonstrated ELISE's superior analytical capabilities, with a final score of 7.98, significantly surpassing ChatGPT (7.10), SciSpace/Typeset (7.10), Epsilon (6.98) and Humata (6.86). Statistical analyses demonstrated significant differences, reinforcing ELISE's effectiveness in analyzing scientific content compared to other models.

3.5Language change and AI tools efficiency

To evaluate the impact of language on AI tools performances, we assessed their ability to process identical articles written in French and English (Article 6 and Article 7 - Lowry et al., 2023a) . The evaluation was conducted using the Extraction, Comprehension, and Analysis criteria, and the results are presented in Figure 6 .

Performance scores for the Extraction criterion revealed a greater variability across languages. ChatGPT achieved 8.06 in French and 7.78 in English, ELISE 6.67 and 8.33, Epsilon 4.56 and 4.00, Humata 0.00 and 3.61, and SciSpace/Typeset 1.11 and 1.94, respectively. These results suggest that ChatGPT and Epsilon maintained stable performance across both languages, while Humata and ELISE exhibited better results in English.

For Comprehension and Analysis criteria, performance variability across languages was minimal for all AI tools, with no strong preference for one language over the other, except for Humata, which consistently performed better in English. The comprehension scores were 7.25 and 7.21 for ChatGPT, 8.38 and 8.63 for ELISE, 8.17 and 7.94 for Epsilon, 6.67 and 7.88 for Humata, 7.63 and 7.42 for SciSpace/Typeset. The analysis criterion followed a similar trend with 7.33 and 6.94 for ChatGPT, 8.28 and 7.92 for ELISE, 7.50 and 6.94 for Epsilon, 7.11 and 8.06 for Humata, 7.67 and 7.83 for SciSpace/Typeset.

These findings highlight that language influences Extraction performance more than Comprehension and Analysis. While some AI tools perform equally well across languages, others exhibit discrepancies, particularly in data retrieval tasks, emphasizing the need for further linguistic adaptation in AI-driven scientific analysis.

3.6Human expertise and AI tools

To further examine AI tools' capabilities, we assessed their performance in Comprehension and Analysis criteria without providing human-validated reference answers. The goal was to evaluate how AI tools perform autonomously when interpreting scientific texts, and the results are presented in Figure 7 .

When comparing AI tools' global evaluation scores with and without human expertise as a reference, ELISE consistently demonstrated the highest alignment with expert-level responses. For Comprehension, ELISE's score remained stable (8.32-8.42), whereas other tools demonstrated greater variations: Epsilon (7.56-8.00), ), SciSpace/Typeset (7.43-7.76) and Humata (6.66 and 6.82). In the Analysis criterion, ELISE also maintained minimal variation (7.98-8.13), outperforming ChatGPT (7.10-7.81), SciSpace/Typeset (7.10-7.52), ) and Humata (6.86-6.87).

Notably, ChatGPT, Epsilon, and SciSpace/Typeset exhibited the largest score increases when human expertise was not used as a A multi-model evaluation ensuring unbiased AI scoring -Global average of AI tools' evaluation by AI Evaluator models (Claude 3.5 Sonnet, GPT-4o, o1 Preview and the average global score (Global) for the Comprehension and Analysis criteria applied to Article 5 (Jones et al., 2021). Comparison between identified and anonymized AI tool response.

Frontiers in Artificial Intelligence 09 frontiersin.org reference, with variations ranging from 0.3 to 0.9 points. These results suggest that AI evaluators models might overestimate AI-generate answers with a more lenient AI tools-assessments considering also some of the evaluators (GPT-4o and o1-Preview) self-assess by scoring ChatGPT answers. In contrast, ELISE consistently produced reliable responses, with minimal variation between the two evaluation settings, reinforcing its alignment with expert-level reasoning. Humata followed a similar trend, but with significantly lower scores, indicating less overall accuracy and robustness compared to ELISE.

To further validate these observations, specific cases where AI tools exhibited string discrepancies were analyzed. The results are visually represented in Figures 8 9 10 .

In the first case (Figure 8 ), AI tools were required to extract Event-Free Survival (EFS) percentages at 60 months from a Kaplan-Meier curve. Epsilon, Humata and SciSpace/Typeset failed to generate relevant responses, while ChatGPT provided incorrect values (21 and 30% instead of 37 and 44%). In contrast, ELISE generated the closest approximation (40 and 50%) and explicitly indicated a margin of error, demonstrating a more expert-like approach to data interpretation.

In another example (Figure 9 ), AI tools had to identify the numbers of excluded articles based on specific selection criteria.

While SciSpace/Typeset was unable to provide an exact number, ChatGPT, Epsilon and Humata misinterpret the exclusion criteria, leading to incorrect responses. Only ELISE successfully differentiated between exclusion categories and provided the correct answer (12 articles excluded due to missing full text, demonstrating its superior ability to recognize complex selection criteria and accurately extract relevant numerical data.

The third case (Figure 10 ) required AI tools to identify a Hazard Ratio (HR) for a specific population within a data table, necessitating both vertical and horizontal reading to locate the expected value. ELISE was the only AI tool capable of retrieving the correct HR (0.31, 95% CI: 0.13-0.74). Moreover, it provided a response even more precise than the expected answer, showcasing its advanced document parsing capabilities and its ability to accurately interpret structured data, a task where all other AI tools failed.

These findings (Figures 7-10 and Supplementary Tables 1 2 3 ) reinforce that ELISE is the AI tool that aligns most closely with human expertise across all tested evaluation criteria. Unlike other models, which exhibited greater variability and inconsistencies, ELISE consistently provided responses that matched expert expectations, particularly in challenging tasks involving graph interpretation, inlay detection, and the comprehension of complex scientific data.

3.7Overall results

To provide a comprehensive overview of the AI tools' evaluation, a detailed comparison of scores across criteria (Figure 11A ) and an overall averaged comparison (Figure 11B ) were conducted.

The results confirm that ELISE consistently achieved high scores across all criteria, demonstrating minimal variation between Extraction, Comprehension and Analysis.

Among the evaluated tools, ChatGPT performed best in Extraction but exhibited lower efficiency in Comprehension and Analysis, indicating its strength in retrieving structured metadata but its relative weakness in processing and interpreting scientific content. In contrast, SciSpace/Typeset, Humata and Epsilon showed poor performance in Extraction but performed moderately better in Comprehension and Analysis, although their results remained less relevant and less consistent than ELISE's.

The global average comparison further reinforces these observations. ELISE emerges as the most effective AI tool, followed by ChatGPT, then SciSpace/Typeset, Humata and Epsilon, which obtained similar but lower overall scores. These findings underscore the importance of an AI tool's ability to handle the entire research workflow, from accurate data extraction to in-depth comprehension and critical analysis, ensuring its reliability for scientific literature processing across various fields and study types.

3.8Statistical analysis

To validate the observed performance differences among AI tools, a comprehensive statistical analysis was conducted. Results for each criterion were included in the global average evaluation (Figures 2F, 4F, 5F ).

A unidirectional ANOVA test (data not shown) confirmed that AI tool performance varied significantly depending on the evaluation criterion, with some tools excelling in certain task underperforming in others. Additionally, two-way ANOVA tests (data not shown) demonstrated that AI tools differed significantly from each other across all criteria, confirming that no single evaluation metric can fully determine an AI tool's effectiveness in scientific literature analysis.

Interestingly, results indicated no significant interaction effect between AI tools and evaluation criteria, meaning that performance rankings remained consistent regardless of the assessment category. This supports the robustness of the conclusions drawn in this study and validates the methodological soundness of the evaluation framework.

3.9ECACT score

To ensure a rigorous and holistic evaluation framework, an ECACT score was developed, incorporating the Extraction, Comprehension and Analysis criteria alongside two additional dimensions: Compliance and Traceability. These complementary criteria are essential to assessing an AI tool's reliability and adherence to scientific best practices.

The Compliance criterion evaluates whether an AI tool follows predefined guidelines and exclusively relies on documented content to generate responses. The traceability criterion assesses the tool's ability to highlight the relevant data sources that were used to generate its answers. These criteria provide a more nuanced understanding of each AI model's transparency and scientific rigor.

To reflect the importance of each criterion, a weighting system was applied. The Analysis criterion received the highest weight, followed by Comprehension, then Extraction. Similarly, Traceability was weighted equivalently to Analysis, while Compliance was weighted at the same level as Comprehension, ensuring a balanced assessment of AI tools' capabilities.

The results, presented in Figure 12 , show that ELISE (Figure 12B ) demonstrated the highest performance across all evaluated criteria, extraction, structuring complex information, and streamlining regulatory and scientific documentation. These tools leverage Named Entity Recognition (NER) to efficiently identify key data points, reducing manual workload and accelerating critical decision-making processes. However, despite these advantages, significant challenges remain, particularly in ensuring the reliability, accuracy, and contextual relevance of AI-generated outputs. Many traditional AI struggle to interpret complex technical content, leading to misinterpretations, inconsistencies, and errors in data extraction. These limitations highlight the need for AI solutions capable of handling industry-specific requirements, where precision, compliance, and traceability are essential for regulatory submissions, clinical trials, and scientific validation.

To address these limitations, the integration of Retrieval-Augmented Generation (RAG) with Large Language Models (LLMs) offers a promising solution for improving accuracy, reliability, and compliance in AI-driven document analysis. By combining advanced retrieval mechanisms with context-aware generation, this approach reduces dependence on pre-trained datasets, which may contain biases or outdated information, and instead ensures fact-based, real-time content generation (Mostafapour et al., 2024;Doyal et al., 2023). One of the most persistent challenges in regulated industries like pharma and Medtech remains the parsing of unstructured documents, including clinical trial reports, regulatory filings, and research publications. In this study, ELISE's superior performance, closely aligned with human expertise, can be attributed to its advanced parsing capabilities. Unlike other AI tools, ELISE demonstrated a higher efficiency in processing text, formula and table, surpassing industry alternatives such as Megaparse, Llama or Unstructured. Preliminary study demonstrated a Normalized Edit Distance (NED) -how different two elements are by counting the minimum changes (insertions, deletions, substitutions) needed to transform one into the other, normalized by the longest element's length with 0 as identical and 1 completely different-respectively of 0.558241, 0.622417, 0.574559, and 0.490463 for Megaparse, Llama, Unstructured and Matsu (ELISE parser developed by Biolevate) on the global performance metric for textual content. When combined with context-aware modeling, this feature enhances query interpretation, ensure more precise responses and improve data traceability, making ELISE particularly adapted to regulatory and clinical applications.

The reliability of AI-generated outputs remains a key concern for industries where compliance with regulatory framework is non-negotiable. One striking example from this study (detailed in Supplementary Figure 2 ) highlights a critical issue: ChatGPT provided a DOI for an article when no other AI tools succeeded, but further investigation revealed that this DOI was not present in the original FIGURE 12 ECACT score evaluation and AI tool comparison. (A-E) Radar charts illustrating AI tools' performance across all evaluation criteria. (F) Global ECACT scores, confirming ELISE's superior reliability and effectiveness across all dimensions. frontiersin.org document, ChatGPT had sourced it externally, violating strict data integrity guidelines. Such discrepancies underscore the importance of traceability and compliance features in AI tools, particularly in clinical research, regulatory submissions, and drug development workflows, where data provenance must be verifiable and reproducible.

To ensure a rigorous and unbiased evaluation of AI tools, a reference human answer was established to benchmark AI-generated responses. To further mitigate evaluation biases, AI Evaluator models were incorporated into the scoring methodology. By leveraging LLMs trained on diverse datasets, these evaluators ensure that responses are analyzed based on factual accuracy rather than subjective human biases. This multi-model validation approach minimizes overestimated AI-generated responses and ensures a more reliable assessment of AI performance, particularly in regulatory and clinical settings.

AI tools such as ChatGPT demonstrate remarkable capabilities in processing large volumes of data, offering significant advantages in speed and accessibility. However, their lack of industry-specific knowledge and contextual awareness makes human oversight essential in critical applications. This study addressed this gap by using expert-defined reference answers, ensuring AI-generated content meets the highest standards of accuracy and relevance for pharmaceutical, biotechnological, and medical applications. Moreover, the ethical and regulatory implications of AI-driven research and documentation must be considered, particularly concerning biases, transparency, and compliance with industry standard. While AI can augment scientific workflow, it cannot replace human expertise. Instead, a hybrid model, where AI supports human decision-making while ensuring data integrity and compliance, offers the most reliable and scalable approach for integrating AI into regulated industries (Mehta et al., 2024;Mostafapour et al., 2024;Ahaley et al., 2024;Singh et al., 2024).

One of the key differentiators among AI tools is compliance and traceability. AI solutions like ELISE integrate built-in compliance mechanisms, allowing for systematic verification of extracted data and response relevance. Unlike general-purpose AI models, which lack transparent methodologies, ELISE explicitly highlights source data and provides traceability on how each response was generated. This feature is critical for regulatory bodies and compliance teams in the pharma, biotech, and Medtech industries, where decision-making must be based on verifiable evidence rather than opaque AI-generated summaries. Furthermore, AI tools capable of explaining their reasoning processes, such as ELISE, allow human experts to refine AI queries, optimize search strategies, and improve model training over time, making them more aligned with human expertise (as demonstrated in Supplementary Figure 3 ).

Despite advancements in Deep Neural Networks (DNNs), Natural Language Processing (NLP), and Transformers architectures, AI models still struggle with high-level analytical reasoning, contextual variation, and long-form document coherence. These challenges impact critical decision-making in pharmaceutical and medical research, where AI-generated insights must be reliable, interpretable, and reproducible. To address these gaps, state-of-the-art techniques such as Pointer-Generator Networks and Sparse Attention Transformers are being implemented to enhance scientific summarization, improve structured data interpretation, and extract meaningful insights from large-scale regulatory of clinical documents (Tsirmpas et al., 2024).

Given the specialized needs of pharma, biotech, and Medtech, AI tools should be designed with modular adaptability, allowing organizations to select and integrate the most suitable models for their specific applications. As AI becomes more deeply embedded in regulatory, clinical, and research workflows, standardized industry guidelines must be established to ensure transparency, compliance, and ethical AI deployment. Human oversight will continue to play a critical role in refining AI-generated insights, ensuring scientific validity, and maintaining alignment with industry regulations, reinforcing the value of a hybrid AI-human approach in optimizing research and clinical decision-making (Bran et al., 2024;Meyer-Szary et al., 2024). AI tools that provide explainability, such as ELISE, play a crucial role in enhancing human-AI collaboration. By offering transparency on how responses are generated, these tools enable users to understand the AI's reasoning process, refine their queries for more precise outputs, and iteratively train the model to align more closely with expert-level expectations (as detailed in the Supplementary Figure 3 ). This capability is particularly valuable in regulated environments such as pharmaceuticals, biotechnology and Medtech, where interpretability, compliance, and continuous model improvement are essential for integrating AI into decisionmaking workflows.

4.1ECACT score and standardized AI evaluation in regulated industries

The integration of AI tools into pharmaceutical, clinical, and healthcare workflow presents both significant opportunities and operational challenges. While AI optimizes processes such as medical documentation, literature review and regulatory reporting, the variability in AI-generated content quality necessitates a standardized evaluation framework.

Existing regulatory framework, such as SPIRIT-AI and CONSORT-AI, provide essential guidance for AI-driven clinical trials, ensuring transparency, reproducibility, and accountability (McGenity and Treanor, 2021). However, they do not provide standardized methodologies for assessing AI-generated research and regulatory outputs. Similarly, the PRISMA 2020 Checklist, widely used for systematic reviews, lacks specific AI assessment criteria (Page et al., 2021).

To address this gap, this study introduces a dedicated AI evaluation framework based on three core criteria: Extraction, Comprehension, and Analysis, each associated with a structured set of questions. This approach allows progressive assessment from basic data retrieval to advanced contextual analysis, ensuring AI tools are evaluating on their full operational capacity.

Additionally, data transparency in AI-generated response is a critical factor in regulatory compliance. As highlighted in the Danler study, AI models must be assessed not only on their accuracy but also on their ability to justify and trace their responses to verifiable sources. The variability in response quality further reinforces the importance of integrating Compliance and Traceability into AI evaluations (Danler et al., 2024). frontiersin.org While ECACT is not a clinical trial reporting guideline like SPIRIT-AI or CONSORT-AI, it complements these frameworks by offering a performance-based evaluation tool specifically for AI-driven document analysis. Future versions of ECACT may integrate harmonization elements with these standards for broader interoperability.

By incorporating these dimensions into the ECACT score, this study demonstrates that AI tools like ELISE, which follow compliance protocols and ensure traceability, significantly outperform models that do not. For instance, Epsilon, which frequently violates guidelines and generates overly verbose responses, was found to be less reliable despite strong comprehension scores. Similarly, ChatGPT's inability to provide traceable references led to its reassignment from second to last position when these additional criteria were included.

While ChatGPT demonstrates lower performance in traceability and analysis under the ECACT framework, it consistently ranks high in fluency, syntactic clarity, and readability. These traits make it a valuable option for use cases outside regulatory or high-stakes contexts, such as educational summaries, internal research notes, or exploratory drafts. ECACT should thus be interpreted as a scenario-sensitive evaluation tool, guiding tool selection according to task constraints.

Moving forward, the ECACT score should evolve to incorporate additional rating scales to further refine Compliance and Traceability assessments. Moreover, ethical considerations-including data privacy, processing speed, and AI model resource consumptionmust be integrated into AI evaluation frameworks. By establishing a standardized, transparent evaluation methodology, as proposed in this study, AI-driven research and regulatory applications can be optimized while ensuring data integrity and ethical compliance (Danler et al., 2024;Wattanapisit et al., 2023;Tangsrivimol et al., 2025).

The current study is limited to life science and biomedical articles. The sample size (n = 9) was deliberately kept small to allow for detailed, multi-criteria assessment of each tool's performance. However, this limited scale restricts the broader generalizability of the results. Future work will focus on validating the ECACT framework on independent datasets, non-English corpora beyond French-English, and diverse scientific domains such as engineering and social sciences, using larger and more heterogeneous article corpora.

Future versions of ECACT may integrate quantitative metrics of semantic similarity (e.g., cosine distance, ROUGE, BERTScore) to complement evaluator-based assessments and further reduce subjectivity in open-ended tasks.

5Conclusion

The study assessed the performance of AI tools in scientific literature analysis, focusing on Extraction, Comprehension, and Analysis criteria while also introducing Compliance and Traceability as critical evaluation dimensions. ELISE emerged as the most effective tool, demonstrating superior performance across all criteria, particularly in data extraction and analytical reasoning, aligning closely with human expertise. ChatGPT exhibited strong efficiency in data retrieval but struggled with deeper comprehension and analysis, limiting its applicability for highly regulated environments. Epsilon, Humata, and SciSpace/Typeset performed moderately, with notable strengths in comprehension but significant weaknesses in structured data extraction, impacting their reliability for complex scientific and regulatory applications.

A key takeaway from this study, is that human oversight remains indispensable in validating AI-generated content, ensuring accuracy, compliance, and contextual relevance, particularly in pharmaceutical, biotechnological, and Medtech applications where data integrity and regulatory adherence are paramount. While AI tools significantly enhance efficiency in literature analysis and knowledge extraction, they must function as augmentative tools rather than standalone solutions.

To address the variability in AI-generated responses and provide a structured evaluation framework, this study introduced the ECACT score, incorporating Extraction, Comprehension, Analysis, Compliance, and Traceability, as key performance indicators. This scoring system ensures that AI tools are assessed not only for their ability to process scientific content but also for their transparency, adherence to guidelines, and ability to justify their outputs. Moving forward, establishing standardized evaluation frameworks such as ECACT will be crucial for integrating AI-driven solutions into research, clinical, and regulatory environments, ensuring that these tools meet the highest standards of scientific rigor, reliability, and ethical compliance.

Data availability statement

The original contributions presented in the study are publicly available. This data can be found here: https://github.com/Biolevate/ SL-EVAL-ECACT.

annex

A significant shift in AI tools rankings was observed when Compliance and Traceability were factored into the evaluation, further reinforcing the need for transparency and guideline adherence in AI-driven research tools.

Notably, ChatGPT's performance declined significantly due to its lack of traceability, while Epsilon's overall score decreased due to its failure to comply with evaluation guidelines. Conversely, ELISE remained consistently at the top (Figure 12F ), demonstrating that it is not only the most performant AI tool for scientific literature analysis but also the most reliable in terms of transparency and methodological rigor.

3.10Sensitivity analysis of the ECACT score

To assess the robustness of the ECACT framework, a sensitivity analysis was performed by modifying the relative weights of each criterion (e.g., equal weights; Compliance and Traceability prioritized). While minor fluctuations were observed in the ranking of mid-performing tools, the top and bottom positions remained consistent. ELISE systematically outperformed other tools across all tested schemes (see Supplementary Table 2 and Supplementary Figure 4 ), confirming the resilience of the ECACT methodology.

4 Discussion and future directions AI tools are increasingly transforming workflows in pharmaceuticals, biotechnology, and Medtech by automating data

Conflict of interest

MaG, MuG, ST, LF, JB, AV, and FA were employed by the Biolevate, the developer of the AI tool ELISE analyzed in this study.

Generative AI statement

The authors declare that Gen AI was used in the creation of this manuscript. This article was partially prepared with the assistance of ELISE, an artificial intelligence tool developed by Biolevate, to support Frontiers in Artificial Intelligence 19 frontiersin.org the literature review, the drafting and structuring of the scientific content.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/frai.2025.1587244/ full#supplementary-material

Funding

FundingThe author(s) declare that no financial support was received for the research and/or publication of this article.

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